Simultaneous equation method for uv spectroscopy
SIMULTANEOUS EQUATION METHOD OR VIERODT'S METHOD If a sample contains two absorbing drugs (X and Y) each of which absorbs at the λmax different from the other, it may be possible to determine both drugs by the technique of simultaneous equations (Vierodt's method), provided certain criterias apply. The information required is (a) The aborptivities of X at and λ1 and λ2 are ax1 and ax2 respectively (b) The aborptivities of Y at and λ1 and λ2 are ay1 and ay2 respectively. (c) The absorbances of the diluted sample at λ1 and λ2 are A1...