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Showing posts from January 25, 2017

Chromatography and it's types

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Chromatography Chromatography is usually introduced as a technique for separating and/or identifying the components in a mixture. The basic principle is that components in a mixture have different tendencies to adsorb onto a surface or dissolve in a solvent. It is a powerful method in industry, where it is used on a large scale to separate and purify the intermediates and products in various syntheses. Different types of chromatography:- 1.      Thin layer chromatography (TLC) 2.      Gas chromatography (GC) 3.      Liquid chromatography (LC) 4.      High performance liquid chromatography (HPLC) 5.      Ion exchange chromatography 6.      Gel permeation or Gel filtration chromatography . Principles Chromatographic methods require one static part (stationary phase) and one moving part (mobile phase). The techniques rely on one of the follow...