High performance Liquid chromatography(HPLC)
1. Introduction High-Performance Liquid Chromatography (HPLC: formerly referrd to ashigh-pressour liquid Chrometography ), is a techique in analytical chemistry used to separate, identify, and quantify each component in a mixture. The principle of chrometography, in chromatography a liquid is pumped through a bed of particles. The liquid is called the mobile phase and the particle the staionary phase . High performance liquid chrometography high performance liquid chrometography (HPLC) is basicallly a highy improved from liquid chrometography. Instead of solvent being chrometograhy allowed todrip though a column under gravity, it is forced through under high pressur of up to 400 atmospheres.That makes it much faster. All chrometograhy separation , includinHPLC operate under the sane basic principle; sepration of a samll into it’s constituent parts because of the difference in the relative affinities of defferent molecules for the mobile phase an...